Prerequisite Protocols

• Transformed Escherichia coli NEB Turbo cells on a colony plate

Materials

• 70% Ethanol (Ice Cold)
• 100% Isopropanol (Ice Cold)
• Sterile Distilled Water
• LB Broth (Miller Mod) + Plasmid Antibiotics
• A 15mL aliquot of Miniprep Resuspension Buffer (250mL), RNAseA added
• Miniprep Lysis Buffer (250mL)
• Miniprep Neutralization Buffer (250mL)
• Sterile 2mL Round Bottom Tubes
• Sterile 1.5mL Tubes

Equipment

• Incubator Shaker (37°C, 250rpm)
• Microcentrifuge capable of 10,000rcf
• DNA Quantification Instrument
• Static Incubator (37°C; optional but ideal)
• Sterile 125mL Erlenmeyer Flask with breathable foam stopper
• Vortexer
• Pipettes