Ingredients

• Sodium Hydroxide
• Sodium Dodecyl Sulfate 10% Solution

Equipment

• Magnetic Stirrer
• Analytical Balance
• 500mL Beaker
• 250mL Volumetric Flask
• 250mL Storage Bottle

Step By Step

1. To a 500mL beaker add 200mL of distilled water and begin stirring.
2. Add 10g of Sodium Hydroxide powder and allow to dissolve. Final concentration will be 200mM.
3. Add 25mL of SDS 10% solution. You can also measure powder but I DO NOT RECOMMEND WORKING WITH POWDERED SDS. WEAR RESPIRATOR AND DO NOT BREATHE IN DUST. Please just get yourself a 10% stock solution. Please. It’s totally safe soap when in water but can collapse your lungs’ alveoli and you’ll end up in a hospital or worse. Final concentration of the SDS should be 1% v/v.
4. Once all additives are dissolved, slowly pour the contents into a 250mL volumetric flask and top off the flask to the 250mL neck mark with distilled water. Invert GENTLY so you mix the solution but limit bubble formation.
5. Transfer solution to a 250mL bottle with a tight screw cap, label bottle with the date as this lysis buffer is sensitive to atmospheric CO2 and will degrade with time. Ideally make fresh every year.

Notes

• DO NOT WORK WITH POWDERED SDS
• The NaOH in the lysis buffer reacts with CO2 in air and degrades so please keep cap tightly on bottle when not in use.

References

Shared on twitter (X) via J. Leon @jesussaur