Prerequisite Protocols

• none

Materials

• Murashige & Skooge Basal Medium with Gamborg Vitamins (4.44g)
• Sucrose (30g)
• MES Buffer (0.5g)
• KOH 1M (~1mL)
• Distilled Water

Equipment

• Magnetic Stirrer and Stir Bar for 1000mL
• Analytical Balance accurate to 0.001g
• Laminar Flow Hood or Sterile Area (dead air box, bunsen sterile field, etc)
• 2x 500mL Wide Mouth Pyrex Glass Bottles
• 6x 250mL PRE-STERILIZED Narrow Mouth Pyrex Bottles
• 1000mL Beaker
• 1000mL Volumetric Flask
• 500mL Volumetric Flask
• Autoclave or InstantPot (Pressure Cooker)
• pH Meter (optional but ideal) or pH strips
• Autoclave Indicator Tape