Ingredients (needed quantity)

• Yeast Extract (5g)
• Tryptone (10g)
• Sodium Chloride (10g)
• Agar (15g)
• Distilled Water (1000mL)

Equipment

• Magnetic Stirrer and Stir Bar for 1000mL
• Analytical Balance accurate to 0.001g
• Laminar Flow Hood or Sterile Area (dead air box, bunsen sterile field, etc)
• 2x 500mL Wide Mouth Pyrex Glass Bottles
• 6x 250mL PRE-STERILIZED Narrow Mouth Pyrex Bottles
• 1000mL Beaker
• 1000mL Volumetric Flask
• 500mL Volumetric Flask
• Autoclave or InstantPot (Pressure Cooker)
• pH Meter (optional but ideal) or pH strips
• Autoclave Indicator Tape
• Reusable Polypropylene Petri Dishes (PRE-STERILIZED)

Step By Step