Ingredient Vol. (stock solution) (conc.)

• 10mL Tris (1M, ph 7.5) (200mM final)
• 2.5mL NaCl (5M) (250mM final)
• 2.5mL EDTA (0.5M) (25mM final)
• 2.5mL SDS (10%) (0.5% final)
• Distilled Water to 50mL Final Volume

Equipment

• Ability to accurately measure up to 10mL (serological pipette ideally)
• 50mL STERILE Centrifuge Tube

Step By Step

1. Add each of the above mentioned ingredients to a sterile 50mL tube. Use a serological pipette if possible, or multiple repeated dispensings of a 1000uL pipette.
2. Top off the solution to the 50mL mark with sterile distilled water.
3. Invert gently to mix. Do not vortex as SDS will cause heavy foaming.
4. Store at room temp indefinitely.

Notes

Lovely brew. I use it on everything.

References

Arabidopsis gDNA isolation - OpenWetWare

A simple and rapid method for the preparation of plant genomic DNA for PCR analysis - PubMed (nih.gov)