Prerequisite Protocols

Materials

• TAE Buffer 50x Concentrate (10mL)
• Purple Loading Dye 6x (NEB)
• Agarose Powder (1.5g)
• NEB 1KB Plus DNA Ladder
• 50mL Centrifuge Tube
• Distilled Water (1000mL)
• GelGreen DNA Stain 10,000x Concentrate (0.5uL to 1uL)

Equipment

• Microwave Oven
• Volumetric Flask 1000mL
• Graduated Cylinder 200mL
• Bottle to Store 1L of 1x Running Buffer
• Gel Electrophoresis Rig
• 250mL Erlenmeyer Flask w/ Rubber Stopper
• Hot Gloves
• 50mL Tube Holder
• Blue Light DNA Transilluminator w/ Light Filter