Ingredients

• Sodium Hydroxide (200mM final conc)
• Potassium Chloride (120mM final conc)
• EDTA 0.5M pH 8.0 (10mM final conc)
• SDS 10% (0.5% final conc)
• Sucrose (20% w/v final conc)
• Bromophenol Blue (0.001% final conc)
• Distilled Water (250mL final vol)

Equipment

• Analytical Balance
• 50mL Conical Tube
• 50mL storage bottle

Step By Step

1. To a 50mL conical centrifuge tube, add 20mL of distilled water and begin stirring.
2. Add 20g of Sucrose and vortex to fully dissolve.
3. Add 894mg of Potassium Chloride powder.
4. Add 800mg of Sodium Hydroxide pellets and vortex to dissolve remaining solids.
5. Add 2mL of EDTA 0.5M pH 8.0
6. Add 5mL of 10% SDS stock solution.
7. Add 10mg of Bromophenol Blue powder